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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IP IH N

Possible applications include the purification of human gamma Interferon and in vitro neutralization. In general, monoclonal antibodies to gamma Interferon are able to inhibit Schwartzman reactions and in the murine system appear to protect NZB mice against spontaneous development of autoimmune disease.
Catalog #	I7662-16Q
Applications	Can be used for immunoassays, Immunohistology on frozen sections and Immunoprecipitation. Furthermore the antibody is useful for Neutralization of the biological activity of Interferon gamma.
Recommended Dilution	Immunohistology: 1:10
	For neutralization of biological activity in vitro dilutions have to be made according to the amount of IFN-gamma to be inactivated.
	Optimal dilutions to be determined by the researcher.
Unit Definition	One neutralizing unit of anti-human gamma interferon is defined as the amount of antibody sufficient for neutralizing one unit human gamma interferon (ref.: NIH standard Gg23-901-530).
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	6A568
Host	Mouse
Source	Human
Concentration	As reported
Form	Supplied as a lyophilized powder in PBS.
Purity	Purified IgG
Specificity	Binds and neutralizes both natural and recombinant human gamma Interferon. Crossreactivity with other cytokines has not been found. Does not react with rodent interferons.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.