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You are here:Home » Antibodies » Abs to Interferon » Anti -Interferon gamma (IFNg)

Anti -Interferon gamma (IFNg)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH N
IFN-gamma is a pluripotent cytokine with important pro-inflammatory functions.
Catalog #I7662-16T
ApplicationsUseful for Neutralization of human IFN-gamma in bio-assays. Furthermore the antibodies can be used for Western Blot, immunoassays and to Immunohistochemically stain cells containing IFN-gamma in frozen sections as well as in cells cultured on cover slips.
Recommended DilutionImmunohistology: 1:10
Western Blot: 1:10
For neutralization of biological activity in vitro dilutions have to be made according to the amount of IFN-gamma to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, containing 0.02% sodium azide and 0.1% BSA.
PurityPurified IgG
SpecificityReacts with human gamma IFN (IFN-gamma) of both natural and recombinant origin. Inhibits the biological activity of natural and recombinant human IFN-gamma. Specificity was further assessed by ELISA and Western Blot. No crossreactivities with other cytokines have been detected.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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