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You are here:Home » Antibodies » Abs to Interferon » Anti -Interferon gamma (IFNg)

Anti -Interferon gamma (IFNg)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified IP N
Catalog #I7662-16V
ApplicationsCan be used for in vitro and in vivo inhibition of activity of IFN-gamma. Furthermore the antibody is useful for immunoassays and Immunoprecipitation.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Unit DefinitionFor neutralization of biological activity dilutions have to be made according to the amount of Interferon to be inactivated. One neutralizing unit is defined as the amount of antibody sufficient for neutralizing one unit of mouse gamma Interferon antiviral activity as calibrated against NIH standard Gg02-901-533.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No6A566
HostRat
SourceMouse
ConcentrationAs reported
FormSupplied as a lyophilized powder in PBS.
PurityPurified IgG
SpecificityBinds and neutralizes both natural and recombinant mouse gamma Interferon. Its binding and neutralizing activity has been demonstrated in vitro and in vivo. F3 antibodies have been demonstrated to be able to inhibit inflammatory responses to bacterial lipopolysaccharides. These antibodies were furthermore shown to inhibit Shwartzman reactions and to protect NZB mice against spontaneous development of autoimmune disease. Does not react with rat or human gamma interferon.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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