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Specifications

Clone Host Grade Applications Monoclonal Rat Affinity Purified IP

Monoclonal antibody F3 binds and neutralizes both natural and recombinant mouse gamma Interferon. Its binding and neutralizing activity has been demonstrated in vitro and in vivo. F3 antibodies have been demonstrated to be able to inhibit inflammatory responses to bacterial lipopolysaccharides. These antibodies were furthermore shown to inhibit Shwartzman reactions and to protect NZB mice against spontaneous development of autoimmune disease.
Catalog #	I7662-17Q
Applications	Suitable for use in Immunoassays and Immunoprecipitation. Other applications not tested.
Recomended Dilution	For neutralization of biological activity dilutions have to be made according to the amount of Interferon to be inactivated. One neutralizing unit is defined as the amount of antibody sufficient for neutralizing one unit of mouse gamma Interferon antiviral activity as calibrated against NIH standard Gg02-901-533.
	Storage and Stability:
	Lyophilized powder may be stored at-20°C. Stable for 12 months at -20°C. Reconstitute with 0.5 ml sterile distilled or de-ionized water. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	9G196
Host	Rat
Source	Mouse
Concentration	As reported
Form	Supplied as a lyophilized powder in PBS. Reconstitute the vial by injection of 0.5 ml sterile distilled or de-ionized water (Caution: vial is under vacuum).
Purity	Purified
Immunogen	Mouse IFNg.
Specificity	IFNg binds and neutralizes both natural and recombinant mouse gamma Interferon. The antibody does not react with rat or human gamma interferon.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names	IFN-gamma