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Specifications

Clone Host Grade Applications Monoclonal Rat Affinity Purified E

Mature Interferon gamma (IFN-gamma) is a homodimeric cytokine produced primarily by T-lymphocytes and natural killer cells. IFN-gamma has many immunoregulatory and proinflammatory activities. It binds to IFN-gamma receptors present on virtually all cell types. The 17kD mature bovine IFN-gamma shares 97% aa sequence identity with ovine IFN-gamma and 77%-80% with canine, equine, and porcine IFN-gamma. It shares 61%, 40%, and 42% identity with human, mouse, and rat IFN-gamma, respectively.
Applications:
Suitable for use in ELISA. Other applications not tested.
Catalog #	I7662-17Q2
Recommended Dilution	ELISA: 2-8ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	9H136
Host	Rat
Source	Bovine
Concentration	~500ug/ml
Form	Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Recombinant bovine IFN-gamma, aa24-166; NP_776511 (E. coli).
Specificity	Recognizes bovine IFN-gamma.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.