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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B

Catalog #	I7662-18C6
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	ELISA: 0.1-0.4ug/ml.
	Western Blot: 0.1-0.2ug/ml. Detection limit for recombinant equine IFN-gamma is ~2ng/lane under non-reducing and reducing conditions.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile TBS containing 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Equine
Concentration	~0.05mg/ml (after reconstitution)
Form	Supplied as a powder lyophilized from 0.2um sterile-filtered solution in PBS containing 50ug BSA per 1ug antibody. Reconstitute with 1ml sterile TBS (20mM Trizma base, 150mM NaCl), pH 7.3, containing 0.1% BSA.
Purity	Purified by immunoaffinity chromatography
Immunogen	Recombinant equine interferon gamma (E. coli)
Specificity	Recognizes equine IFN-gamma
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.