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You are here:Home » Antibodies » Abs to Interferon » Anti -Interferon gamma (IFNg)

Anti -Interferon gamma (IFNg)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B N
Catalog #I7662-18E
Applications
Suitable for use in Neutralization, ELISA and Western Blot. Other applications have not been tested.
Recommended DilutionNeutralization: To yield one-half maximal inhibition [ND50] of the biological activity of rat IFN-gamma (0.3ng/ml), a concentration of 0.0060.01ug/ml of this antibody is required.
ELISA To detect rat IFN-gamma by direct ELISA (using 100ul/well antibody solution) a concentration of at least 0.5ug/ml of this antibody is required. This antigen af nity puri ed antibody, in conjunction with compatible secondary reagents, allows the detection of 0.20.4ng/well of recombinant rat IFN-gamma.
Western Blot To detect rat IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.10.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat IFN-gamma is 1.53ng/lane, under either reducing or non-reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of OriginUSA
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceRat
Concentration~1mg/ml
FormSupplied as a lyophilized powder from a sterile-filtered solution in PBS, pH 7.4.
PurityPurified by antigen affinity chromatography.
ImmunogenHighly purified, recombinant Interferon gamma (IFNg)
SpecificityRecognizes Interferon gamma (IFNg)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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