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Anti -Interferon-inducible T Cell Alpha Chemoattractant, Human (I-TAC) (Biotin)
Anti -Interferon-inducible T Cell Alpha Chemoattractant, Human (I-TAC) (Biotin)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B N |
|
| Catalog # | I7662-19A | | Applications | Suitable for use in ELISA, Western Blot, and neutralization. Other applications have not been tested. | | Recommended Dilution | Neutralization: To yield one-half maximal inhibition [NND50] of the biological activity of hI-TAC (100 ng/ml), a concentration of 2.0-3.0 ug/ml of this antibody is required. | | ELISA | To detect hI-TAC by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.15-0.30ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2ng/well of recombinant hI-TAC. | | Western Blot | To detect hI-TAC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hI-TAC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. | | Optimal dilutions to be determined by the researcher. | | Reconstitution | Use sterile PBS solution with 0.1% BSA to a concentration of 50ug/ml. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 25% glycerol and store at -20°C. May be stored at 4°C for short-term only. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Country of Origin | USA | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a lyophilized powder from a sterile-filtered solution in PBS, pH 7.4. Conjugated to Biotin. | | Purity | >98% Purified by antigen affinity chromatography. | | Immunogen | Highly purified, recombinant Interferon-inducible T Cell Alpha Chemoattractant, Human (I-TAC) | | Specificity | Recognizes Interferon-inducible T Cell Alpha Chemoattractant, Human (I-TAC) | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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