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You are here:Home » Antibodies » Abs to Interferon » Anti -Interferon-inducible T Cell Alpha Chemoattractant, Human (I-TAC) (Biotin)

Anti -Interferon-inducible T Cell Alpha Chemoattractant, Human (I-TAC) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B N
Catalog #I7662-19A
ApplicationsSuitable for use in ELISA, Western Blot, and neutralization. Other applications have not been tested.
Recommended DilutionNeutralization: To yield one-half maximal inhibition [NND50] of the biological activity of hI-TAC (100 ng/ml), a concentration of 2.0-3.0 ug/ml of this antibody is required.
ELISA To detect hI-TAC by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.15-0.30ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2ng/well of recombinant hI-TAC.
Western Blot To detect hI-TAC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hI-TAC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.
ReconstitutionUse sterile PBS solution with 0.1% BSA to a concentration of 50ug/ml.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 25% glycerol and store at -20°C. May be stored at 4°C for short-term only. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of Origin USA
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a lyophilized powder from a sterile-filtered solution in PBS, pH 7.4. Conjugated to Biotin.
Purity>98% Purified by antigen affinity chromatography.
ImmunogenHighly purified, recombinant Interferon-inducible T Cell Alpha Chemoattractant, Human (I-TAC)
SpecificityRecognizes Interferon-inducible T Cell Alpha Chemoattractant, Human (I-TAC)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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