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Interferon gamma, Recombinant, Human (IFNg)
Interferon gamma, Recombinant, Human (IFNg)
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| Human interferon-gamma (hIFN-gamma) is secreted by lymphocytes stimulated by mitogen and is involved in the differentiation, maturation, and proliferation of hematopoietic cells. Natural human interferon-gamma is composed of 143 amino acid residues without cysteine residues and is glycosylated. Recombinant human interferon gamma was expressed in E. coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. Additional amino acid (Methionine) is attached at N-terminus. | | | | | | Molecular Weight: | | | 16.9kD (144 amino acids) | | | | | | Sequence: | | | MQDPYVKEAE/ NLKKYFNAGH/ SDVADNGTLF/ LGILKNWKEE/ SDRKIMQSQI/ VSFYFKLFKN/ FKDDQSIQKS/ VETIKEDMNV/ KFFNSNKKKR/ DDFEKLTNYS/ VTDLNVQRKA/ IHELIQVMAE/ LSPAAKTGKR/ KRSQMLFRGR/ RASQ | | | Catalog # | I7662-21 | | Applications | Suitable for use in ELISA. Other applications not tested. | | Recommended Dilution | Optimal dilutions to be determined by the researcher. | | Endotoxin Level | < 1.0EU/1ug protein (LAL method) | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 16.9kD | | Source | Recombinant, Human, E. coli | | Purity | 95% by SDS PAGE | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 20mM Tris, pH 7.4. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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