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You are here:Home » Growth Factors, Cytokines » Growth Factors-Interferon » Interferon gamma, Recombinant, Human (IFNg)

Interferon gamma, Recombinant, Human (IFNg)

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Specifications

Human interferon-gamma (hIFN-gamma) is secreted by lymphocytes stimulated by mitogen and is involved in the differentiation, maturation, and proliferation of hematopoietic cells. Natural human interferon-gamma is composed of 143 amino acid residues without cysteine residues and is glycosylated. Recombinant human interferon gamma was expressed in E. coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. Additional amino acid (Methionine) is attached at N-terminus.
Molecular Weight:
16.9kD (144 amino acids)
AA Sequence:
MQDPYVKEAE/ NLKKYFNAGH/ SDVADNGTLF/ LGILKNWKEE/ SDRKIMQSQI/ VSFYFKLFKN/ FKDDQSIQKS/ VETIKEDMNV/ KFFNSNKKKR/ DDFEKLTNYS/ VTDLNVQRKA/ IHELIQVMAE/ LSPAAKTGKR/ KRSQMLFRGR/ RASQ
Catalog #I7662-21
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight16.9kD
SourceE. coli
Purity95% by SDS PAGE
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. Endotoxin:
1.0EU/1ug protein (LAL method).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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