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You are here:Home » Growth Factors, Cytokines » Growth Factors-Interferon » Interferon tau, Recombinant, Ovine (IFNt)

Interferon tau, Recombinant, Ovine (IFNt)

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Specifications

Interferon-tau is also known as TP-1 (trophoblast protein-1) is a new class of type I IFN that is secreted by the trophoblast and is the signal for maternal recognition of pregnancy in sheep. Interferon-tau has potent immunosuppressive and antiviral activities similar to other type I IFN but is less cytotoxic than IFN-alpha and IFN-beta. The current investigation concerns the effect of recombinant ovine IFN- tau (Recombinant Ovine IFN- tau) on the modulation of MHC class I and II expression on cloned mouse cerebrovascular endothelial (CVE) cells. IFN-tau induced tyrosine phosphorylation of Stat1 and upregulated the expression of MHC class I on CVE. One proposed action by which type I IFN reduces the relapse rate in MS is via interference with IFN-g-induced MHC class II expression. Interferon tau was shown to downregulate IFN-gamma-induced MHC class II expression on CVE and, hence, may be of potential therapeutic value in downregulating inflammation in the central nervous system (CNS). Interferon- tau did not upregulate the expression of MHC class II on CVE. Interferon- tau also inhibited the replication of Theiler's virus in CVE.
Catalog #I7662-70A1
DescriptionRecombinant Ovine Interferon-Tau produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 172 amino acids and having a molecular mass of ~20kD. Recombinant Ovine IFN-Tau is purified by proprietary chromatographic techniques.
Amino-Acid SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Cys-Tyr-Leu-Ser-Arg.
Dimers and AggregatesLess than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological ActivityOvine IFN-Tau is fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 5.7 x 107 IU/mg.
Endotoxin 0.1ng/ug (IEU/ug) of Recombinant Ovine Interferon-Tau.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.027 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of Recombinant Ovine IFN-Tau as a Reference Standard.
Gene Name:
IFNT1
SolubilityIt is recommended to reconstitute the lyophilized interferon-Tau in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
StabilityLyophilized Recombinant Ovine Tau Interferon although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution IFN-Tau should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Molecular Weight~20kD
SourceE. coli
Purity95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder in 0.5X PBS, pH 7.4.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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