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You are here:Home » Antibodies » Abs to Interferon » Anti -IRF1 (Interferon Regulatory Factor 1, IRF-1, MAR)

Anti -IRF1 (Interferon Regulatory Factor 1, IRF-1, MAR)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
IRF1 (interferon regulatory factor 1) is a 45kD member of the IRF family of proteins. It is induced by TLR9 and INF-g stimulation, and positively regulates the expression of IFN-b, iNOS and 1L-12 p35. Human IRF1 is 325aa in length. It contains an N-terminal DNA-binding basic region aa1-140 and an acidic C-terminus aa141-325. Lys275 and 299 can be SUMOylated, which blocks activity, or ubiquinated at Lys299, which initiates degradation. There is a splice variant that shows an 11aa substitution for the C-terminal 86aa. Over aa171-325, human IRF1 shares 83aa identity with both canine and porcine IRF1.
Catalog #I7662-81F
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2B
Clone No11n89
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to aa147-325 from human IRF1 expressed in E. coli (P10914).
SpecificityRecognizes human IRF1. No cross-reactivity with recombinant human IRF2 (aa151-349), IRF3 (aa206-427), IRF4 (aa130-451), IRF5 (aa214-395), IRF6 (aa176-366), IRF7 (aa289-503), IRF8 (aa255-426), IRF9 (aa238-393), or recombinant mouse IRF1 (aa147-329).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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