You are here:Home
»
Antibodies
»
Abs to Interferon
»
Anti -IRF1 (Interferon Regulatory Factor 1, IRF-1, MAR)
Anti -IRF1 (Interferon Regulatory Factor 1, IRF-1, MAR)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B |
|
| IRF1 (interferon regulatory factor 1) is a 45kD member of the IRF family of proteins. It is induced by TLR9 and INF-g stimulation, and positively regulates the expression of IFN-b, iNOS and 1L-12 p35. Human IRF1 is 325aa in length. It contains an N-terminal DNA-binding basic region aa1-140 and an acidic C-terminus aa141-325. Lys275 and 299 can be SUMOylated, which blocks activity, or ubiquinated at Lys299, which initiates degradation. There is a splice variant that shows an 11aa substitution for the C-terminal 86aa. Over aa171-325, human IRF1 shares 83aa identity with both canine and porcine IRF1. | | | Catalog # | I7662-81F | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 0.5ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2B | | Clone No | 11n89 | | Host | Mouse | | Source | Human | | Concentration | Not determined | | Form | Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Recombinant corresponding to aa147-325 from human IRF1 expressed in E. coli (P10914). | | Specificity | Recognizes human IRF1. No cross-reactivity with recombinant human IRF2 (aa151-349), IRF3 (aa206-427), IRF4 (aa130-451), IRF5 (aa214-395), IRF6 (aa176-366), IRF7 (aa289-503), IRF8 (aa255-426), IRF9 (aa238-393), or recombinant mouse IRF1 (aa147-329). | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
