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You are here:Home » Antibodies » Abs to Interferon » Anti -Interferon Regulatory Factor 3, phosphorylated (Ser386) (IRF3)

Anti -Interferon Regulatory Factor 3, phosphorylated (Ser386) (IRF3)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IC
A family of interferon (IFN) regulatory factors (IRFs) have been shown to play a role in transcription of IFN genes as well as IFN-stimulated genes. The IRF-3 gene encodes a 50-kD protein that binds specifically to the IFN- stimulated response element (ISRE) (1).IRF-3 is activated through phosphorylation by the kinases IKK epsilon and/or TBK1.Phosphorylation results in IRF-3 dimerization and removal of an autoinhibitory structure to allow interaction with the coactivators CBP/p300. Phosphorylation at Serine 396 through Serine 405 alleviates autoinhibition to allow interaction with CREB-binding protein and facilitates phosphorylation at Serine 385 or Serine 386 (2).Serine 386 is suggested to be the target of the IRF-3 kinase and critical determinant for the activation of IRF-3 (3).
Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Catalog #I7662-86H
Recommended DilutionWestern Blot: 1:1000-1:10,000
Immunocytochemistry: 1:250-1:500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No9H411
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 50% glycerol
PuritySupernatant
ImmunogenA phospho-specific peptide corresponding to residues surrounding Serine 386 of human IRF3.
SpecificityRecognizes human IRF3 phosphorylated on Serine 386 at ~47kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesIRF-3


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