You are here:Home
»
Antibodies
»
Abs to Interferon
»
Anti -Interferon Regulatory Factor 3, phosphorylated (Ser386) (IRF3)
Anti -Interferon Regulatory Factor 3, phosphorylated (Ser386) (IRF3)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
B IC |
|
| A family of interferon (IFN) regulatory factors (IRFs) have been shown to play a role in transcription of IFN genes as well as IFN-stimulated genes. The IRF-3 gene encodes a 50-kD protein that binds specifically to the IFN- stimulated response element (ISRE) (1).IRF-3 is activated through phosphorylation by the kinases IKK epsilon and/or TBK1.Phosphorylation results in IRF-3 dimerization and removal of an autoinhibitory structure to allow interaction with the coactivators CBP/p300. Phosphorylation at Serine 396 through Serine 405 alleviates autoinhibition to allow interaction with CREB-binding protein and facilitates phosphorylation at Serine 385 or Serine 386 (2).Serine 386 is suggested to be the target of the IRF-3 kinase and critical determinant for the activation of IRF-3 (3). | | | | | | Applications: | | | Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. | | | Catalog # | I7662-86H | | Recommended Dilution | Western Blot: 1:1000-1:10,000 | | Immunocytochemistry: 1:250-1:500 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 9H411 | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 50% glycerol | | Purity | Supernatant | | Immunogen | A phospho-specific peptide corresponding to residues surrounding Serine 386 of human IRF3. | | Specificity | Recognizes human IRF3 phosphorylated on Serine 386 at ~47kD. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | IRF-3 |
External Links
|
