| This gene encodes a member of the interferon regulatory transcription factor (IRF) family. Family members share a highly-conserved N-terminal helix-turn-helix DNA-binding domain and a less conserved C-terminal protein-binding domain. Mutations in this gene can cause van der Woude syndrome and popliteal pterygium syndrome. This protein is involved in palate formation. |
|
| Catalog # | I7662-89Q |
| Applications | Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested. |
| Recommended Dilutions | |
| Peptide ELISA Titer: 1:1:32,000 |
| Immunohistochemistry: 2-3ug/ml in paraffin embedded Human Kidney shows strong nuclear staining in DCT and glomerulus |
| Western Blot: 0.2-0.6ug/ml; ~60kD band observed in Human Ovary lysates (calculated MW of 53.1kD according to NP_006138.1). In transfected HEK293 transiently expressing IRF6 a band of approx. 55kD is observed. This band is not observed in the non-transfected HEK293 |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Source | Human |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide. |
| Purity | Purified by peptide affinity chromatography. |
| Immunogen | Synthetic peptide corresponding to C-TPSMQLPPALPPQ, from the C Terminus of human IRF6 (NP_006138.1). |
| Specificity | Recognizes human IRF6. Species sequence homology: mouse. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | OFC6, VWS, PPS, PIT, LPS, interferon regulatory factor 6, IRF6 |
