Anti -Interferon Stimulating Gene 15 (ISG15, 15kD ubiquitin-like modifier GIP2, IFI15,
Ubiquitin Cross-reactive Protein, UCRP)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Affinity Purified |
E B IP |
|
| Interferon Stimulating Gene 15 (ISG15) is a member of the ubiquitin-like protein family and functions in various biological pathways from pregnancy to innate immune responses. Expression of ISG15 is stimulated by cellular exposure to type 1 interferons alpha and beta, in addition to infection with viruses such as influenza B. After exposure to type I interferons, both lymphocytes and monocytes, in addition to some fibroblasts and epithelial cells, release ISG15 into culture medium. ISG15 has been shown to function as a cytokine, stimulating interferon gamma secretion by monocytes and macrophages, proliferation of natural killer cells, and chemotactic responses in neutrophils. ISG15 has also been shown to function intracellularly, being covalently conjugated to other proteins by E1 (Ube1L), E2 (UbcH8) and E3 ligases via a multi-step process analogous to ubiquitination. ISG15 is removed from proteins by the ubiquitin processing protease Ubp43. ISG15-protein conjugation (ISGylation) is induced by type 1 interferons, and target proteins include the serine protease inhibitor Serpin 2A, PLC gamma1, ERK1/2, Jak1 and Stat1. Unlike ubiquitination, ISGylation does not target proteins for degradation, rather ISGylation increases Jak1 and Stat1 activity, enhancing the cellular response to interferons. | | | Catalog # | I7662-94A | | Applications | Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000 | | Immunoprecipitation: 1:25 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 7H28 | | Host | Rabbit | | Source | Human | | Concentration | Not determined. | | Form | Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol. | | Purity | Purified by Protein A and peptide affinity chromatography. | | Immunogen | Synthetic peptide corresponding to human Interferon Stimulating Gene 15 (ISG15). | | Specificity | Recognizes endogenous levels of the uncleaved precursor form of human ISG15 protein at ~15kD. Does not recognize the activated (cleaved) or conjugated forms of ISG15. Does not crossreact with other ubiquitin family members, including ubiquitin, SUMO-1, SUMO-2, SUMO-3 or NEDD8. Species crossreactivity: equine. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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