Anti -Interferon Stimulating Gene 15 (ISG15, 15kD Ubiquitin-like Modifier GIP2, G1P2,
Interferon Induced 15kD Protein, IFI15, Interferon alpha Inducible Protein, Interferon
Stimulated Protein 15kD, Ubiquitin Cross-reactive Protein, UCRP)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Purified |
E B |
|
| ISG15 a 15kDa homologue shows no amino acid sequence homology to cytokines and is synthesized as a precursor that is activated through processing by a thiol protease. ISG15 is secreted by monocytes and lymphocytes. Synthesis is induced in response to IFN alpha or IFN beta, but not IFN gamma. ISG15 expression is induced also by overexpression of some interferon regulatory factors that have been shown to play a role in the transcriptional regulation of IFN genes. ISG15 is secreted also by cell lines of monocyte (U937 cell line), T-lymphocyte, B-lymphocyte (DAUDI cells), human fibroblasts, and epithelial origins. The induction of terminal differentiation in human melanoma cells is associated, among other things, with alterations in the expression of ISG15. Intracellularly ISG15 has been shown to function as a ubiquitin homologue. It is known also as UCRP (ubiquitin cross-reactive protein). Serpin 2a (spi2a), a member of the serine protease inhibitor (serpin) protein family that is highly induced in macrophages during bacillus Calmette-Guerin infection has been shown to bind ISG15. ISG15 has been shown to modulate immune cell function. It possesses activities of cytokines and induces production of IFN gamma. It enhances proliferation and functions of natural killer and LAK cells. | | | Catalog # | I7662-94L | | Cellular Localization | Cytoplasmic and Secreted | | Positive Control | Most human cell lysates can be used as a positive control without induction or stimulation. | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~5mg/ml | | Form | 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7 | | Purity | Purified | | Immunogen | Recombinant ISG15(Human). | | Specificity | Species Crossreactivity: Crossreacts with Human. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | Interferon Induced 17kD Protein |
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