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You are here:Home » Molecular Biology » Growth Factors-Interleukin » Interleukin 1 alpha, Recombinant, Rat (IL-1a, Hematopoietin-1, Lymphocyte-activating Factor, LAF, Endogenous Pyrogen, Leukocyte Endogenous Mediator, LEM, Mononuclear Cell Factor, MCF)

Interleukin 1 alpha, Recombinant, Rat (IL-1a, Hematopoietin-1, Lymphocyte-activating
Factor, LAF, Endogenous Pyrogen, Leukocyte Endogenous Mediator, LEM, Mononuclear Cell
Factor, MCF)

Pricing

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Specifications

IL-1alpha is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine. Recombinant rat IL-1 alpha is a 17.7 kD protein containing 155 amino acid residues.
Catalog #I7663-01A
Purity 98% by SDS-PAGE and HPLC analyses
Form The sterile ltered solution was lyophilized from 0.5x Tris buffer saline, pH 8.0.
Endotoxin 0.1ng/ug (1EU/ug)
Biological Activity The ED50 as determined by the dose-dependent proliferation of murine D10S cells is < 0.005ng/ml, corresponding to a speci c activity of > 2 x 109units/mg.
AA Sequence  MAPHSFQNNL RYKLIRIVKQ EFIMNDSLNQ NIYVDMDRIH LKAASLNDLQ LEVKFDMYAY SSGGDSKYPV TLKVSNTQLF VSAQGEDKPV LLKEIPETPK LITGSETDLI FFWEKINSKN YFTSAAFPEL LIATKEQSQV HLARGLPSMI DFQIS
Stability The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted IL-1 alpha should be stored in working aliquots at-20°C.
Reconstitution We recommend a quick spin followed by reconstitution in water to a concentration of 0.11.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or-20°C for future use.
Country of Origin  USA
SourceE. coli
ConcentrationAs reported
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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