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You are here:Home » Antibodies » Abs to Interleukin » Anti -Interleukin 1 alpha (IL-1a)

Anti -Interleukin 1 alpha (IL-1a)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B N
IL-1alpha is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine.
Catalog #I7663-01B
ApplicationsSuitable for use in Neutralization, ELISA and Western Blot. Other applications not tested.
Recommended DilutionNeutralization:To yield one-half maximal inhibition [ND50] of the biological activity of rat IL-1 alpha (50pg/ml), a concentration of 0.0110.017u/ml of this antibody is required.
ELISA To detect rIL-1 alpha by direct ELISA (using 100ul/well antibody solution) a concentration of at least 0.5ug/ml of this antibody is required. This antigen af nity puri ed antibody, in conjunction with compatible secondary reagents, allows the detection of 0.20.4ng/well of recombinant rat IL-1 alpha.
Western Blot To detect rat IL-1 alpha by Western Blot analysis this antibody can be used at a concentration of 0.10.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rIL-1 alpha is 1.53.0ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. May be stored at 4°C for short-term only. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of OriginUSA
Clone TypePolyclonal
IsotypeIgG
HostRat
SourceGoat
Concentration>0.2mg/ml
FormSupplied as a lyophilized powder (1mg/ml), from 0.5X PBS pH 7.4.
PurityPuri ed by af nity chromatography employing immobilized rIL-3 alpha matrix.
ImmunogenHighly pure (> 98%) recombinant rat IL-1 alpha.
SpecificityRecognizes rat IL-1 alpha.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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