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Anti -Interleukin 1 alpha (IL-1a) (Biotin)
Anti -Interleukin 1 alpha (IL-1a) (Biotin)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B |
|
| IL-1alpha is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine. | | | Catalog # | I7663-01C | | Applications | ELISA: To detect rat IL-1-alpha by direct ELISA (using 100ul/well antibody solution) this antibody can be used at a concentration of 0.15-0.30ug/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2ng/well of recombinant rat IL-1-alpha. | | Western Blot | To detect rat IL-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat IL-1-alpha is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. | | Stability | The lyophilized antibody is stable for at least 6 months when kept at –20°C. | | Reconstitution | The biotinylated antibody should be reconstituted to a concentration of 50ug/ml with sterile PBS solution containing 0.1% BSA. This solution can be stored at 4°C for at least one month without detectable loss of activity. Frozen aliquots of this solution are stable for at least 6 months when kept at -20°C. Avoid more than one freeze-thaw cycle. | | Country of Origin | USA | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Rat | | Concentration | ~50ug/ml | | Form | Lyophilized from sterile- ltered PBS solution. | | | Purity | Puri ed by af nity chromatography and then biotinylated. | | Immunogen | Highly pure (>98%) recombinant rat IL-1-alpha. | | Specificity | IL-1-alpha | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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