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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B N

Catalog #	I7663-01D1
Applications	Suitable for use in ELISA, Western Blot and Neutralization. Other applications not tested.
Recommended Dilution	ELISA: To detect rIL-1 alpha by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4ng/well of recombinant rIL-1 alpha.
	Western Blot: To detect rIL-1 alpha by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rIL-1 alpha is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
	Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of rIL-1 alpha (50 pg/ml), a concentration of 0.011-0.017ug/ml of this antibody is required.
	Optimal dilutions to be determined by the researcher.
	Reconstitution:
	Reconstitute to 0.2mg/ml with sterile dH2O or sterile 40-50% glycerol for long term storage.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of Origin	USA
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Rat
Concentration	~1mg/ml
Form	Supplied as a lyophilized powder.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Highly pure (> 98%) recombinant rat IL-1 alpha
Specificity	Recognizes rat IL-1 alpha.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.