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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B

Interleukin 1 alpha (IL-1 alpha) is synthesized as a 33 kD precursor that mainly remains intracellular. Cleavage by calpain in keratinocytes, epithelial cells and endotoxin-stimulated peripheral blood mononuclear cells produces a 112 aa, 16 kD propeptide and a 159 aa, 17 kD mature protein. A nuclear localization site in the propeptide allows transit to the nucleus, where either the precursor or the propeptide can activate transcription or alter RNA splicing. This can increase motility or senescence of normal cells or increase apoptosis of tumor cells. Human and mouse IL-1 alpha propeptide share 71% aa identity.
Catalog #	I7663-01E
Applications	Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilution	Western Blot: 1-2ug/ml
	ELISA: 0.5-1ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2b
Clone No	7H235
Host	Mouse
Source	Human
Concentration	As reported
Form	Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile ddH2O or sterile 40-50% glycerol.
Purity	Purified by Protein G affinity chromatography.
Immunogen	E. coli-derived recombinant human IL-1 alpha propeptide (aa1-112) Accession # PO1583
Specificity	Recognizes human IL-1 alpha propeptide.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.