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You are here:Home » Antibodies » Abs to Interleukin » Anti -Interleukin 1 alpha (IL-1a)

Anti -Interleukin 1 alpha (IL-1a)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Interleukin 1 alpha (IL-1 alpha) is synthesized as a 33kD precursor that mainly remains intracellular. Cleavage by calpain in keratinocytes, epithelial cells and endotoxin-stimulated peripheral blood mononuclear cells produces a 112aa, 16kD propeptide and a 159aa, 17kD mature protein. A nuclear localization site in the propeptide allows transit to the nucleus, where either the precursor or the propeptide can activate transcription or alter RNA splicing. This can increase motility or senescence of normal cells or increase apoptosis of tumor cells. Human and mouse IL-1 alpha propeptide share 71% aa identity.
Catalog #I7663-01E
ApplicationsSuitable for use in Western Blot and ELISA. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml. The detection limit for recombinant human IL-1a propeptide is ~5ng/lane under non-reducing and reducing conditions.
Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant human IL-1a propeptide is ~2ng/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No7H235
HostMouse
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile ddH2O
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa1-112 from human
IL-1 alpha propeptide (PO1583), expressed in E.coli.
SpecificityRecognizes human IL-1 alpha propeptide. Does not crossreact with mature forms of recombinant mouse IL-1a or recombinant rat IL-1a.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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