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You are here:Home » Antibodies » Abs to Interleukin » Anti -Interleukin 1 alpha (IL-1a) (Biotin)

Anti -Interleukin 1 alpha (IL-1a) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
IL-1alpha is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine.
Rat IL-1 alpha is a 17.7kD cytokine, which is produced by macrophages.
Catalog #I7663-01J
ApplicationsELISA: 0.15-0.30ug/ml
Western Blotting: 0.1-0.2ug/ml
Optimal working dilutions to be determined by researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceRat
Concentration~50ug/ml
FormLyophilized in PBS. Reconstitute with 1ml sterile PBS containing 0.1% Bovine Serum Albumin.
PurityPurified IgG was prepared from whole serum by affinity chromatography.
ImmunogenRecombinant rat IL-1 alpha.
SpecificityRecognizes rat interleukin-1 alpha.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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