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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B

Rat IL-1 alpha is a 17.7kD cytokine which is produced by macrophages .IL-1alpha is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine.
Catalog #	I7663-01M
Applications	Suitable for use in ELISA, Western Blot. Other applications not tested.
Recommended Dilutions	ELISA: 4ug/ml using 100ul/well to detect a range of 4-250pg/ml of recombinant rat IL1 alpha.
	Western Blot: 1-2ug/ml using rat IL1 alpha at 100ng/lane under non-reducing and reducing conditions. Predicted molecular weight: 31kD.
	Optimal working dilutions to be determined by researcher.
Recommended Secondary Reagents	Donkey Anti Sheep/Goat IgG: HRP conjugate
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	4F4
Host	Mouse
Source	Rat
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.2.
Purity	Purified from tissue culture supernatant using Protein G. Endotoxin level is < 10ng per mg antibody as determined by the LAL method.
Immunogen	Purified recombinant rat IL1 alpha, expressed in E. coli.
Specificity	Recognizes rat interleukin-1 alpha (IL-1 alpha).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.