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You are here:Home » Antibodies » Abs to Interleukin » Anti -Interleukin 1 alpha (IL-1a) (Biotin)

Anti -Interleukin 1 alpha (IL-1a) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
IL-1alpha is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine.
Catalog #I7663-02C
Applications  
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA: Direct ELISA 0.15-0.30ug/ml (using 100ul/well antibody solution). Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2ng/well of recombinant hIL-1-alpha.
Western Blot 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-1-alpha is 1.5-3ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder in PBS. Labeled with biotin. Reconstitution: Reconstitute with PBS, 0.1% BSA. to a concentration of 0.05mg/ml. This solution can then be diluted into other aqueous solutions.
PurityPuri ed by af nity chromatography.
ImmunogenHighly pure (>98%) recombinant hIL-1-alpha (human IL-1-alpha).
SpecificityRecognizes human Interleukin 1 alpha (IL1a).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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