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You are here:Home » Antibodies » Abs to Interleukin » Anti -Interleukin 1 alpha (IL-1a)

Anti -Interleukin 1 alpha (IL-1a)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B N
Catalog #I7663-02C1
ApplicationsSuitable for use in ELISA, Western Blot and Neutralization. Other applications not tested.
Recommended DilutionELISA: To detect hIL-1 alpha by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4ng/well of recombinant hIL-1 alpha.
Western Blot: To detect hIL-1 alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-1 alpha is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-1 alpha (20ng/ml), a concentration of 1.0-2.0ug/ml of this antibody is required.
Optimal dilutions to be determined by the researcher.
ReconstitutionReconstitute to 0.2mg/ml with sterile dH2O or sterile 40-50% glycerol for long term storage.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of OriginUSA
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a lyophilized powder.
PurityPurified by immunoaffinity chromatography.
ImmunogenHighly pure (> 98%) recombinant human IL-1 alpha
SpecificityRecognizes human IL-1 alpha.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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