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You are here:Home » Antibodies » Abs to Interleukin » Anti -Interleukin 1 alpha (IL-1a)

Anti -Interleukin 1 alpha (IL-1a)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified E B IH FC N
IL-1alpha is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells
This antibody is primarily directed against the 17kD human IL-1a. In general,this antibody also detects primate IL-1a in the same formats using similar dilutions. The antiserum does not recognize human IL-1b or Mouse or Rabbit IL-1a. In ELISA formats and other immunoreactive assays, this antibody will recognize both the mature 17kD IL-1a as well as the 31kD IL-1a precursor in either non-denatured (native) or denatured samples.
Unlike the IL-1b precursor, the native precursor of IL-1a is recognized by the antibody produced to the 17kD form. The
31kD precursor of IL-1a is biologically active and is found primarily intracellularly. The precursor of IL-1a, unlike that of IL-
1b, is biologically active when applied to cells and is thought to have a role as a functional molecule intracellularly and can be
found constitutively expressed in various cell. This antibody is also useful for neutralization of human and primate IL-1a
activity in bioassays. It does not neutralize the biological activity IL-1b. It does not neutralize the biological activity of mouse,
rat or rabbit IL-1a. It will neutralize primate IL-a.
Catalog #I7663-02H
ApplicationsSuitable for use in Neutralizations, ELISA, Immunohistochemistry, Flow Cytometry and Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:100-1:200
ELISA: 1:1000-1:5000
Neutralization: Incubate the sample with a 1:100
dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is
recommended.
Optimal dilution determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
PurityPurified by DEAE fractionation.
ImmunogenRecombinant human IL-1a produced in E. coli
SpecificityRecognizes the 17kD human IL-1a. Crossreactivity: See protocol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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