| IL-1alpha is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells |
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| This antibody is primarily directed against the 17kD human IL-1a. In general,this antibody also detects primate IL-1a in the same formats using similar dilutions. The antiserum does not recognize human IL-1b or Mouse or Rabbit IL-1a. In ELISA formats and other immunoreactive assays, this antibody will recognize both the mature 17kD IL-1a as well as the 31kD IL-1a precursor in either non-denatured (native) or denatured samples. |
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| Unlike the IL-1b precursor, the native precursor of IL-1a is recognized by the antibody produced to the 17kD form. The |
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| 31kD precursor of IL-1a is biologically active and is found primarily intracellularly. The precursor of IL-1a, unlike that of IL- |
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| 1b, is biologically active when applied to cells and is thought to have a role as a functional molecule intracellularly and can be |
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| found constitutively expressed in various cell. This antibody is also useful for neutralization of human and primate IL-1a |
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| activity in bioassays. It does not neutralize the biological activity IL-1b. It does not neutralize the biological activity of mouse, |
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| rat or rabbit IL-1a. It will neutralize primate IL-a. |
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| Catalog # | I7663-02H |
| Applications | Suitable for use in Neutralizations, ELISA, Immunohistochemistry, Flow Cytometry and Western Blot. Other applications not tested. |
| Recommended Dilution: |
| Western Blot: 1:500-1:2000 |
| Immunohistochemistry: 1:100-1:200 |
| ELISA: 1:1000-1:5000 |
| Neutralization: Incubate the sample with a 1:100 |
| dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is |
| recommended. |
| Optimal dilution determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added. |
| Purity | Purified by DEAE fractionation. |
| Immunogen | Recombinant human IL-1a produced in E. coli |
| Specificity | Recognizes the 17kD human IL-1a. Crossreactivity: See protocol. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
