| Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of the interleukin 2 gene. NFAT binds to a sequence in the interleukin 2 gene enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the smaller of which is the product of this gene. The encoded protein binds strongly to the 90 kDa protein and stimulates its ability to enhance gene expression. |
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| Catalog # | I7663-04 |
| Applications | Suitable for use in ELISA, Western Blot, Immunofluorescence. Other applications not tested. |
| Recommended Dilution | Peptide ELISA Titer: 1:4000 |
| Western Blot: 0.3-1ug/ml. ~45kD band observed in Human Tonsil and Thymus lysates. |
| Immunofluorescence: 2-3ug/ml. Paraformaldehyde-fixed stable knock-down cell line (second half) shows merely background compared to the wildtype cell line (left half). Data kindly provided by Dr. A Antes, UMDNJ, Newark, USA. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Peptide Blocking | Corresponding peptide is available for Peptide Blocking studies. See I7663-04-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Host | Goat |
| Source | Human |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide. |
| Purity | Purified by affinity chromatography. |
| Immunogen | A synthetic peptide from the C-terminus of aa--EKAYEKPPEKKEG |
| Specificity | Recognizes human Interleukin enhancer binding factor 2. Species Crossreactivity: mouse, rat and canine. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | ILF2; interleukin enhancer binding factor 2, 45kDa; MGC8391; NF45; PRO3063; interleukin enhancer binding factor 2; nuclear factor of activated T-cells, 45-kDa |
