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You are here:Home » Molecular Biology » Growth Factors-Interleukin » Interleukin 1, alpha, Recombinant, Human (IL-1a, Hematopoietin-1, Lymphocyte-activating Factor, LAF, Endogenous Pyrogen, Leukocyte Endogenous Mediator, LEM, Mononuclear Cell Factor, MCF)

Interleukin 1, alpha, Recombinant, Human (IL-1a, Hematopoietin-1, Lymphocyte-activating
Factor, LAF, Endogenous Pyrogen, Leukocyte Endogenous Mediator, LEM, Mononuclear Cell
Factor, MCF)

Pricing

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Specifications

IL-1 alpha is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine.
Catalog #I7663-07E
Recombinant human IL-1 alpha is a single non-glycosylated polypeptide chain containing 159 amino acids corresponding to aa113-271.
AA SequenceSAPFSFLS NVKYNFMRII KYEFILNDAL NQSIIRANDQ YLTAAALHNL DEAVKFDMGA YKSSKDDAKI TVILRISKTQ LYVTAQDEDQ PVLLKEMPEI PKTITGSETN LLFFWETHGT KNYFTSVAHP NLFIATKQDY WVCLAGGPPS ITDFQILENQ A
Biological ActivityFully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of murine D10S cells is less then 0.001ng/ml, corresponding to a Specific Activity of 1 x 10e9 IU/mg.
Endotoxin 1EU/ug as determined by LAL method.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~18kD
SourceEscherichia coli
Purity~97% by SDS-PAGE and HPLC analyses.
Concentration~1mg/ml
FormSupplied as a lyophilized powder from PBS, pH 7.5. Reconstitute in sterile ddH2O or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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