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Interleukin 10, Recombinant, Human (IL-10)
Interleukin 10, Recombinant, Human (IL-10)
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| Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of a-helical cytokines that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155 . IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts . Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells . IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kD noncovalently associated homodimer . The IL-10 dimer binds to two IL-10 Ra/IL-10R1 chains, resulting in recruitment of two IL-10 Rb/IL-10R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 . IL-10Rb does not bind IL-10 by itself but is required for signal transduction . IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation . It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses. | | | Catalog # | I7663-07N | | Biological Activity | Determined by the dose-dependent co-stimulation (with murine IL-4) of MC/9 cells. The ED50 was found to be 2ng/ml, corresponding to a specific activity of 5 x 10e5 units/mg. | | Endotoxin | 1EU/ug as determined by LAL method. | | Storage and Stability | Aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months. | | Molecular Weight | 18.6kD | | Source | Escherichia coli | | Purity | ~95% by SDS-PAGE and HPLC analyses. | | Form | Supplied as a lyophilized powder in PBS, pH 7.5. Reconstitute in sterile ddH2O or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1mg/ml. | | . | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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