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You are here:Home » Antibodies » Antibodies-Interleukin » Anti -Interleukin 1 beta (IL-1b)

Anti -Interleukin 1 beta (IL-1b)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B N
Catalog #I7663-11
ApplicationsSuitable for use in ELISA, Western Blot and Neutraliztion. Other applications not tested.
Recommended DilutionElisa (Indirect, Sandwich): 0.5-2.0ug/ml. Detects 0.2-0.4ng/well of recombinant rat IL-1-beta. (using 100ul/well antibody solution).
Western Blot: 0.1-0.2ug/ml. Detects recombinant rat IL-1-beta at 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Neutralization: 0.09-0.14ug/ml. Yields one-half maximal inhibition [ND50] of the biological activity of rat IL-1-beta (0.2ng/ml).
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of OriginUSA
Clone TypePolyclonal
ConcentrationNot determined
FormSupplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 40-50% glycerol, ddH2O to a concentration of 0.1-1mg/ml.
PurityPuri ed by immobilized antigen af nity chromatography.
ImmunogenHighly pure (>98%) recombinant rat IL-1-beta.
SpecificityRecognizes rat IL-1-beta.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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