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You are here:Home » Antibodies » Abs to Interleukin » Anti -Interleukin 1 beta (IL-1b)

Anti -Interleukin 1 beta (IL-1b)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IC N
Interleukin-1B (IL-1beta) is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. Human IL-1beta is a 17kD protein containing 153 amino acid residues.
Catalog #I7663-11C
ApplicationsSuitable for use in ELISA, Western Blot, Immunocytochemistry and Neutralization of Bioactivity. Other applications not tested.
Recommended DilutionsWestern Blot: 0.1ug/ml using recombinant human IL-1b
Immunocytochemistry: 5-15ug/ml using immersion fixed human peripheral blood lymphocytes and immersion fixed human peripheral blood mononuclear cells treated with LPS and monensin.
Neutralization: 0.004-0.00ug/ml in the presence of 50pg/ml recombinant human IL-1b and 1.25ug/ml concanavalin A. Measured by its ability to neutralize IL 1 b-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder in PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by immunoaffinity chromatography. Endotoxin: 0.01EU/ug (LAL)
ImmunogenRecombinant protein corresponding to aa117-269 from human IL-1b expressed in E. coli (NP_000567).
SpecificityRecognizes human IL-1 beta. In Direct ELISAs, ~30 75% crossreactivity with recombinant mouse IL 1b, recombinant porcine IL-1b, recombinant rat IL-1b, recombinant canine IL-1b, recombinant equine IL-1b, recombinant cotton rat IL-1b, recombinant feline IL 1b, recombinant Rhesus monkey IL-1b and recombinant guinea pig IL-1b.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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