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You are here:Home » Antibodies » Abs to Interleukin » Anti -Interleukin 1 beta (IL-1b)

Anti -Interleukin 1 beta (IL-1b)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH N
Interleukin-1B (IL-1beta) is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. Human IL-1beta is a 17kD protein containing 153 amino acid residues.
Catalog #I7663-11C
ApplicationsSuitable for use in ELISA, Western Blot, Immunohistochemistry and Neutralization of Bioactivity. Other applications not tested.
Recommended DilutionDirect ELISA: 0.5-1ug/ml. Detection limit for rhIL-1b is ~0.15ng/well.
Western Blot: 0.1-0.2ug/ml. Detection limit for rhIL-1b is ~0.5ng/lane under both non-reducing and reducing conditions.
Immunohistochemistry (Frozen): 5-15ug/ml. Detects human IL-1b in cultured cells or tissue sections.
Optimal dilutions to be determined by the researcher.
Neutralization of Human IL-1b BioactivityThe exact concentration of antibody required to neutralize rhIL-1b activity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. To provide a guideline, the neutralization dose for this antibody can be determined under a specific set of conditions. The Neutralization Dose 50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 for this lot of I7663-11C was determined to be ~0.004-0.008ug/ml in the presence of 50pg/ml of recombinant human IL-1b using a D10.G4.1 cell proliferation assay.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
PurityPurified by immunoaffinity chromatography. Endotoxin: 0.01Eu/1ug (LAL)
ImmunogenRecombinant corresponding to human IL-1b expressed in E. coli.
SpecificityRecognizes human IL-1 beta. Neutralizes the biological activity of recombinant human IL-1b and natural human IL-1b. Will not neutralize the biological activity of recombinant mouse IL-1b, recombinant human IL-1a, or recombinant mouse IL-1a. Based on direct ELISA results, this antibody shows less than 10% crossreactivity with recombinant mouse IL-1b, recombinant porcine IL-1b, recombinant rat IL-1b, recombinant crIL-1b and recombinant rfeIl-1b.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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