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You are here:Home » Antibodies » Antibodies-Interleukin » Anti -Interleukin 1 beta (IL-1b)

Anti -Interleukin 1 beta (IL-1b)


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Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IC N
Interleukin-1B (IL-1beta) is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. Human IL-1beta is a 17kD protein containing 153 amino acid residues.
Catalog #I7663-11C
ApplicationsSuitable for use in ELISA, Western Blot, Immunocytochemistry and Neutralization of Bioactivity. Other applications not tested.
Recommended DilutionsWestern Blot: 0.1ug/ml using recombinant human IL-1b
Immunocytochemistry: 5-15ug/ml using immersion fixed human peripheral blood lymphocytes and immersion fixed human peripheral blood mononuclear cells treated with LPS and monensin.
Neutralization: 0.004-0.00ug/ml in the presence of 50pg/ml recombinant human IL-1b and 1.25ug/ml concanavalin A. Measured by its ability to neutralize IL 1 b-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder in PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by immunoaffinity chromatography. Endotoxin: 0.01EU/ug (LAL)
ImmunogenRecombinant protein corresponding to aa117-269 from human IL-1b expressed in E. coli (NP_000567).
SpecificityRecognizes human IL-1 beta. In Direct ELISAs, ~30 75% crossreactivity with recombinant mouse IL 1b, recombinant porcine IL-1b, recombinant rat IL-1b, recombinant canine IL-1b, recombinant equine IL-1b, recombinant cotton rat IL-1b, recombinant feline IL 1b, recombinant Rhesus monkey IL-1b and recombinant guinea pig IL-1b.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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