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You are here:Home » Antibodies » Abs to Interleukin » Anti -Interleukin 1 beta (IL-1b)

Anti -Interleukin 1 beta (IL-1b)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Highly Purified E B IP IH
Interleukin-1b is produced by activated macrophages, IL-1B stimulates thymocyte proliferation by inducing il-2 release, b-cell maturation and proliferation, and fibroblast growth factor activity. IL1B proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Catalog #I7663-11G
ApplicationsSuitable for use in Direct ELISA, Western Blot, Immunoprecipitation, Inhibition and Immunohistochemistry. Other applications not tested.
Recommended DilutionsDirect ELISA: 1:20,000 will yield >1.0 O.D. using alkaline phosphatase conjugated rabbit anti-mouse Ig.
Inhibition: 1:2000 will completely inhibit a thymocyte co-stimulation assay using suboptimal doses of concanavalin A.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
IsotypeIgG2b
Clone No5C57
HostMouse
SourceHuman
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder in PBS, pH 7.2. Reconstitute with 500ul of sterile ddH2O. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
PurityPurified by Ion Exchange chromatography
ImmunogenRecombinant protein corresponding to human IL-1b
SpecificityRecognizes human IL-1b
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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