| Interleukin-1 beta (IL-1beta) is a potent immunomodulator which mediates a wide range of immune and inflammatory responses, including the activation of B and T cells. Murine IL-1b is a 17.5kD protein containing 153 amino acid residues. |
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| Catalog # | I7663-11K |
| Applications | Suitable for use in ELISA, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. |
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| Recommended Dilutions: |
| Flow Cytometry: 1:10. Use 10ul to label 10e6 cells in 100ul. Membrane permeabilization is required. |
| Immunohistochemistry: Frozen sections. |
| Optimal dilutions to be determined by researcher. |
| Recommended Secondary Antibodies | I1904-65W: IgG, F(ab’)2 (HRP) Pab Rb xMo |
| I1904-66A: IgG, F(ab’)2 (FITC) Pab Rb xMo |
| Recommended Negative Control | I1904-78R: IgG1 Murine Negative Control |
| Recommended Pair | Capture: I7663-11K |
| Detection: I7663-10E |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H2506 |
| Host | Mouse |
| Source | Sheep |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. |
| Purity | Purified |
| Immunogen | Recombinant ovine IL-1 Beta. |
| Specificity | Recognizes sheep interleukin-1 Beta and shows no crossreactivity with sheep IL-6, IL-8, TNF alpha or MCP-1. Species Crossreactivity: goat, equine and bovine. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
