| IL-1 beta (also known as Interleukin-1 beta, IL-1ß and catabolin) is produced by activated macrophages. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. |
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| Catalog # | I7663-11N |
| Applications | Suitable for use in RIA, ELISA, Western Blot, Neutralization, Immunoprecipitation and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | ELISA: 1:500-1:2000 Best used as the second antibody in combination with a monoclonal antibody as a capture antibody. |
| Western Blot: 1:1000 Supernatants or lysates of 2x10e6 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1%human serum plus 10ng/ml E.coli LPS. |
| NOTE: However, in immunoblot analysis of natural cell products or human body fluids, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31kD IL- 1b precursor molecule. Denatured 31kD precursor IL-1b will be recognized by this antibody but often migrates as a 35kD band. This is due to the unfolding of the denatured precursor IL-1b exposing epitopes not exposed in the natural state. |
| Immunohistochemistry (Paraffin fixation, frozen): 1:200- |
| Immunoprecipitation: 1:400-1:800 Pre-clearing with a non-specific rabbit IgG is |
| helpful to reduce background. |
| Neutralization: (Primate IL-1b) 1:200 >4hours |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | Not determined |
| Form | Supplied as a liquid in 0.02M potassium phosphate, pH 7.2, 0.15M sodium chloride. |
| Purity | Serum |
| Immunogen | Recombinant human IL-1b produced in E. coli, 153aa, 17kD with the N-terminal amino acid at position alanine 117. This cleavage site is generated by the IL-1b converting enzyme (ICE, capase-1). |
| Specificity | Recognizes the 17kD mature Human IL-1b. Does not recognize human IL-1a. Recognizes 10% of the non-denatured (native) precursor 31kD IL-1b containing samples but will primarily detect all of the 17kD mature molecule. Also useful for neutralization of human and primate IL-1b activity in bioassays. It does not neutralize the biological activity IL-1a. It does not neutralize the biological activity of murine, rat or rabbit IL-1b. Species Crossreactivity: Canine, primate. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
