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You are here:Home » Antibodies » Abs to Interleukin » Anti -Interleukin 1 beta (IL-1b) (Biotin)

Anti -Interleukin 1 beta (IL-1b) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Interleukin-1beta (IL-1beta), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses. It is produced primarily by activated monocytes and macrophages. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets. Human IL-1beta is synthesized as a 31 kD precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kD mature form. Detection of the 17 kD mature form of IL-1beta is a good indicator of caspase-1 activity.
Catalog #I7663-13
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionDirect ELISA: 0.15-0.30ug/ml using 100ul/well. Allows the detection of at least 0.2ng/well.
Western Blot: 0.1-0.2ug/ml. The detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
ConcentrationNot determined
FormSupplied as a lyophilized powder from PBS. Labeled with Biotin. Reconstitute with sterile ddH2O.
PurityPuri ed by af nity chromatography.
ImmunogenHighly pure (>98%) recombinant rat Interleukin-1-beta.
SpecificityRecognizes rat Interleukin 1 beta.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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