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You are here:Home » Molecular Biology » Growth Factors-Interleukin » Interleukin 1 beta, Recombinant, Mouse (IL-1b)

Interleukin 1 beta, Recombinant, Mouse (IL-1b)

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Specifications

A soluble factor produced by monocytes, macrophages, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. Murine macrophages display a transition from IL-1 beta to IL-1 alpha production during maturation of monocytes into inflammatory macrophages. The synthesis of IL-1 can be induced by other cytokines including TNF-alpha, IFN-alpha, IFN-beta and IFN-g and also by bacterial endotoxins, viruses, mitogens, and antigens. In human skin fibroblasts IL-1 alpha and TNF-alpha induce the synthesis of IL-1beta. In pheochromocytoma cells, NGF induces the synthesis of IL-1.
Catalog #I7663-17A
Recombinant Murine IL-1 is a non-glycosylated, Polypeptide chain containing 153 amino acids and having a molecular mass of 17.5kD.
Amino Acid SequenceMVPIRQLHYR LRDEQQKSLV LSDPYELKAL HLNGQNINQQ VIFSMSFVQGEPSNDKIPVA LGLKGKNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRFVFNKIEVKSK VEFESAEFPN WYISTSQAEH KPVFLGNNSG QDIIDFTMES VSS
Biological ActivityMurine Interleukin-1 beta is fully biologically active when compared to standard. The ED50 is <2pg/ml, corresponding to a Specific Activity of 5x10e8 IU/mg.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C after receipt . Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight17.5kD
SourceE. coli
Purity98% by RP-HPLC, SDS-PAGE. Chromatographically purified.
FormSupplied as a lyophilized powder from PBS, pH 7.5. Reconstitute with sterile ddH2O to 0.1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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