Interleukin 1 beta, Recombinant, Mouse (IL-1b)
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| A soluble factor produced by monocytes, macrophages, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. Murine macrophages display a transition from IL-1 beta to IL-1 alpha production during maturation of monocytes into inflammatory macrophages. The synthesis of IL-1 can be induced by other cytokines including TNF-alpha, IFN-alpha, IFN-beta and IFN-g and also by bacterial endotoxins, viruses, mitogens, and antigens. In human skin fibroblasts IL-1 alpha and TNF-alpha induce the synthesis of IL-1beta. In pheochromocytoma cells, NGF induces the synthesis of IL-1. | | | Catalog # | I7663-17A | | Recombinant Murine IL-1 is a non-glycosylated, Polypeptide chain containing 153 amino acids and having a molecular mass of 17.5kD. | | Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Val-Pro-Ile-Arg. | | Biological Activity | Murine Interleukin-1 beta is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of murine D10S cells < 0.002ng/ml, corresponding to a Specific Activity of 5 x10e9 IU/mg. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O, 0.1% HSA or BSA, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. | | Source | Murine, recombinant protein expressed in E. coli | | Purity | 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: Less than 0.1ng/ug (IEU/ug). | | Form | Supplied as a lyophilized powder in 5mM Tris, pH 8.0, sodium phosphate dibasic.Reconstitute with sterile dH2O, 0.1 HSA or BSA to 0.1mg/mL | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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