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You are here:Home » Molecular Biology » MB-Growth Factors-Interleukin » Interleukin 1 beta, Recombinant, Mouse (Interleukin-1 beta, IL-1 beta, Il1b, Il-1b)

Interleukin 1 beta, Recombinant, Mouse (Interleukin-1 beta, IL-1 beta, Il1b, Il-1b)


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Catalog #I7663-17B
SourceRecombinant corresponding to 153aa of mouse Interleukin-1 beta expressed in E.coli is a non-glycosylated, polypeptide chain.
Molecular Weight~17.5kD
Biological ActivityThe ED50 as determined by the dose-dependent stimulation of mouse D10S cellsis less then 0.002ng/ml, corresponding to a Specific Activity of 5x109 IU/mg.
Dimers and Aggregates 1% (SDS-PAGE)
Protein ContentProtein quantitation was carried out by two independent methods: 1.UV spectroscopy at 280nm 2. Analysis by RP-HPLC, using a standard solution of mouse IL-1 beta as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~17.5kD
Purity~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug)
FormSupplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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