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You are here:Home » Antibodies » Abs to Interleukin » Anti -Interleukin 1 beta (IL-1b)

Anti -Interleukin 1 beta (IL-1b)

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Specifications

Clone Host Grade Applications
Polyclonal Rat Affinity Purified E N
Catalog #I7663-19D
ApplicationsSuitable for use in ELISA and Neutralizeation of IL-1b activity. Other applications not tested.
Recommended DilutionELISA: 2-4ug/ml as a capture antibody
Neutralization: ~1-2ug/ml will neutralize 50% of the bioactivity of 50pg/ml of recombinant mouse Interleukin 1 beta in the presence of 5x10e4 cells/ml.
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRat
SourceMouse
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 5% trehalose, 0.1% sodium azide before the addition of glycerol to 40%.
PurityPurified by Protein G affinity chromatography. Endotoxin level:
<0.1EUg/ug of antibody (LAL)
ImmunogenRecombinant mouse IL-1 beta
SpecificityRecognizes mouse Interleukin 1 beta (IL-1b).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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