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You are here:Home » Antibodies » Abs to Interleukin » Anti -Interleukin 1 beta (IL1b, IL-1 beta, Catabolin, Hematopoietin 1, H1, IFN beta Inducing Factor, IL1F2, LAF, OAF, Osteoclast Activating Factor, Preinterleukin beta, Pro Interleukin 1 beta)

Anti -Interleukin 1 beta (IL1b, IL-1 beta, Catabolin, Hematopoietin 1, H1, IFN beta
Inducing Factor, IL1F2, LAF, OAF, Osteoclast Activating Factor, Preinterleukin beta, Pro
Interleukin 1 beta)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B
IL-1beta, also known as IL1F2, is a prototypical member of the IL-1 superfamily. It is produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. The biological activity of IL-1beta is mediated by the heterodimeric receptor complex containing IL-1 R1 (IL-1 RI) and IL-1 R3 (IL-1 RAcP). The IL-1beta propeptide is cleaved intracellularly by caspase-1/ICE to generate a 17kD active cytokine. An alternatively spliced form of equine IL-1beta lacks the caspase-1 cleavage site and is potentially membrane-associated.
Catalog #I7663-20A
ApplicationsSuitable for use in Western Blot and Direct ELISA. Other applications have not been tested.
Recommended DilutionsWestern Blot: 1-2ug/ml. The detection limit for recombinant equine IL-1b is ~5ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity 5 to 50 fold
Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant equine IL-1b is ~2ng/well.
Optimal dilutions to be determined by the researcher.
HybridomaProduced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from rat.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No8B25
HostRat
SourceEquine
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa116-268 of equine IL-1 beta expressed in E. coli (#Q28386). Species Sequence Homology: 64-72% aa sequence identity with bovine, feline, goat, human, mouse, ovine, porcine, rabbit and rat IL-1beta.
SpecificityRecognizes equine IL-1beta. Does not crossreact with recombinant canine IL-1 beta, recombinant cotton rat IL-1 beta, recombinant feline IL-1 beta, recombinant human IL 1 beta, recombinant porcine IL-1 beta, recombinant rat IL 1 beta, recombinant human IL-1H1, or recombinant mouse IL-17R beta.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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