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You are here:Home » Molecular Biology » Growth Factors-Interleukin » Interleukin 1 beta, Recombinant, Rat (IL-1b)

Interleukin 1 beta, Recombinant, Rat (IL-1b)

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Specifications

Interleukin-1beta (IL-1beta) is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. Rat IL-1 is a 17.3kD protein containing 153 amino acid residues.
Catalog #I7663-23
Source E. coli
Reconstitution Lyophilized rat IL-1beta is soluble in water and most aqueous buffers. The lyophilized protein can be reconstituted in water to a concentration of 0.1-1.0mg/ml. This solution can be diluted into other buffered solutions or stored at- 20°C for future use.
Biological ActivityThe ED50 of Rat IL-1beta as determined by the dose-dependent stimulation of murine D10S cells is  0.1ng/ml, corresponding to a specific activity of  1x10e7 units/mg.
AA Sequence MVPIRQLHCR LRDEQQKCLV LSDPCELKAL HLNGQNISQQ VVFSMSFVQG ETSNDKIPVA LGLKGLNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRF VFNKIEVKTK VEFESAQFPN WYISTSQAEH RPVFLGNSNG RDIVDFTMEP VSS
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile ddH2O, protein carrier. Aliquot and store at -20°C. Reconstituted protein is stable for 3 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight17.3kD
SourceE. coli
Purity98% by SDS-PAGE and HPLC analyses Endotoxin: 0.1ng/ug
FormSupplied as a lyophilized powder from sterile-filtered 4mM Tris, pH 8.0, without additives. Reconstitute with sterile dH2O, carrier protein to concentration of 0.1-1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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