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You are here:Home » Molecular Biology » Growth Factors-Interleukin » Interleukin 1 beta, Recombinant, Rat (IL-1b)

Interleukin 1 beta, Recombinant, Rat (IL-1b)

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Specifications

A soluble factor produced by monocytes, macrophages, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. IL-1 consists of two distinct forms, IL-1 alpha and IL-1 beta which perform the same functions but are distinct proteins. The synthesis of IL-1 can be induced by other cytokines including TNF-alpha , IFN-alpha , IFN-beta and IFN-g and also by bacterial endotoxins , viruses, mitogens, and antigens. In human skin fibroblasts IL-1 alpha and TNF-alpha induce the synthesis of IL-1beta . In pheochromocytoma cells, NGF induces the synthesis of IL-1.
Catalog #I7663-23A
DescriptionRecombinant Rat Interleukin-1 beta produced in E.Coli is a non-glycosylated, Polypeptide chain containing 153 amino acids and having a molecular mass of 17415.82 Dalton. The Recombinant Rat IL-1b is purified by proprietary chromatographic techniques.
Amino Acid SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Val-Pro-Ile-Arg.
Dimers and AggregatesLess than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological ActivityThe ED50 as determined by the dose-dependent stimulation of murine D10S cells is < 0.1 ng/ml, corresponding to a Specific Activity of 1 x 107 IU/mg.
Endotoxin 0.1ng/ug (IEU/ug) of Recombinant Rat Interleukin-1 beta.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.558 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of Rat IL-1b as a Reference Standard.
GeneName: Il1b
SolubilityIt is recommended to reconstitute the lyophilized Rat Interleukin-1b in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
StabilityLyophilized Rat IL-1 beta although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution IL-1 beta should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Molecular Weight17.42kD
SourceE. coli
Purity98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
Concentration~1mg/ml
FormSupplied as a lyophilized powder in 10mM sodium citrate, pH 3.0.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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