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You are here:Home » Antibodies » Abs to Interleukin » Anti -Interleukin 1, Family Member 10 (IL-1F10, Interleukin 1 Receptor Antagonist-like FIL1 theta, Interleukin 1 theta, IL-1 theta, FIL1 theta, Interleukin 1 HY2, IL-1HY2)

Anti -Interleukin 1, Family Member 10 (IL-1F10, Interleukin 1 Receptor Antagonist-like
FIL1 theta, Interleukin 1 theta, IL-1 theta, FIL1 theta, Interleukin 1 HY2, IL-1HY2)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B
Human IL-1F10/IL-1HY2 is a secreted cytokine belonging to the IL-1 family. It is synthesized as a 152 amino acid mature protein that lacks an identifiable signal sequence. It binds to soluble IL-1 R1. Human IL-1F10 shares 82% aa sequence identity with mouse IL-1F10. Human IL-1F10 also shares 39% and 27% aa sequence identity with IL-1ra and IL-1 , respectively.
Catalog #I7663-24S
HybridomaProduced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a rat immunized with purified, E. coli-derived, recombinant human Interleukin 1 family member 10 (rhIL-1F10; aa 1-152; Accession # AAK68048).
ApplicationsWestern Blot, Direct ELISA
Recommended DilutionWestern Blot: This antibody can be used at 1-2ug/mL with the appropriate secondary reagents to detect human IL-1F10. Using a colorimetric detection system, the detection limit for rhIL-1F10 is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold.
Direct ELISA: This antibody can be used at 0.5-1.0ug/mL with the appropriate secondary reagents to detect human IL-1F10. The detection limit for rhIL-1F10 is approximately 2 ng/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No8H144
HostRat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder in PBS, 5% trehalose. Reconstitution: Reconstitute with sterile PBS. If 0.2ml of PBS is used, the antibody concentration will be 500ug/ml.
PurityPurified by Protein G affinity chromatography.
ImmunogenE.coli-derived rhIL-1F10
SpecificityRecognizes human IL-1F10. In direct ELISAs and Western Blots, this antibody shows approximately 50% crossreactivity with rmIL-1F10 and no crossreactivity with rhIL-1 , rhIL-1 , rhIL-1ra, rhIL-18, rhIL-1F5, rhIL-1F6, rhIL-1F7, rhIL-1F8, or rhIL-1F9.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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