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You are here:Home » Antibodies » Abs to Interleukin » Anti -Interleukin 1 beta (IL1b, IL-1 beta, Catabolin, Hematopoietin 1, H1, IFN beta Inducing Factor, IL1F2, LAF, OAF, Osteoclast Activating Factor, Preinterleukin beta, Pro Interleukin 1 beta)

Anti -Interleukin 1 beta (IL1b, IL-1 beta, Catabolin, Hematopoietin 1, H1, IFN beta
Inducing Factor, IL1F2, LAF, OAF, Osteoclast Activating Factor, Preinterleukin beta, Pro
Interleukin 1 beta)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E B IP IH FC
Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types, and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin1 alpha (IL1 alpha) and interleukin1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25% homology in their amino acid sequence.
Catalog #I7663-24V4
Cellular Localization Secreted
ApplicationsSuitable for use in RID, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry (Fr) (paraffin-embedded sections). Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
Form0.02M Potassium Phosphate, 0.15M sodium chloride, pH 7.2
PurityPurified
ImmunogenRecombinant 153 aa human IL-1beta produced in E.coli. MW of recombinant IL-1beta 15kDa, with the N-terminal amino acid at position alanine 117. This is the cleavage site generated by the IL-1beta converting enzyme (ICE, capase-1).
SpecificityPrimarily directed against mature 17kDa human IL-1beta. In ELISA formats and other immunoreactive assays, the antibody will recognize 10% of the non-denatured (native) 31kDa IL-1beta precursor containing samples, but will detect all of the 17kDa mature molecule. Also detects primate IL-1beta in the same formats using similar dilutions. The antiserum does not recognize human IL-1alpha. Species Crossreactivity: Crossreacts with Human and Primate. Does not crossreact with Mouse, Rabbit or Rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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