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You are here:Home » Antibodies » Abs to Interleukin » Anti -Interleukin 1 beta (IL1b, IL-1 beta, Catabolin, Hematopoietin 1, H1, IFN beta Inducing Factor, IL1F2, LAF, OAF, Osteoclast Activating Factor, Preinterleukin beta, Pro Interleukin 1 beta)

Anti -Interleukin 1 beta (IL1b, IL-1 beta, Catabolin, Hematopoietin 1, H1, IFN beta
Inducing Factor, IL1F2, LAF, OAF, Osteoclast Activating Factor, Preinterleukin beta, Pro
Interleukin 1 beta)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B N
Interleukin 1 (IL-1) activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL-1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types, and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin1 alpha (IL-1 alpha) and interleukin1 beta (IL-1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25% homology in their amino acid sequence.
Catalog #I7663-24V5
ApplicationsSuitable for use in ELISA, Western Blot and Neutralization. Other applications not tested.
Recommended DilutionNeutralization: Measured by its ability to neutralize IL­1beta/IL­1F2­induced proliferation in the D10.G4.1 mouse helper T cell line. The Neutralization Dose (ND50) is typically 0.001­0.003ug/ml in the presence of 50pg/ml recombinant Human IL-1beta/IL-1F2 and 1.25ug/ml concanavalin A.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1
Clone No8L427
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2. No preservative added.
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant human IL1 beta (rhIL1 beta), expressed in E. coli.
SpecificityBy ELISA and immunoblotting, the antibody shows < 5% crossreactivity with rhIL1 alpha, rmIL1 beta. In addition the antibody shows no crossreactivity with rmIl1 alpha, rhIL1 ralpha, rmIL1 ralpha, and rrIL1 ralpha. The antibody will not neutralize the biological activity of rhIL1 alpha, rmIL1 alpha or rmIL1 beta. Species Crossreactivity: Crossreacts with Human. The antibody shows < 5% crossreactivity with recombinant mouse IL-1beta.Not yet tested in other species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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