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You are here:Home » Molecular Biology » Growth Factors-Interleukin » Interleukin 1 Receptor Antagonist, Recombinant, Human (IL-1RA, IL1RA, IL-1ra3, IL-1RN, IL1RN, Anakinra, DIRA, ICIL-1RA, IL1 Inhibitor, IL1F3, IRAP, MGC10430, MVCD4)

Interleukin 1 Receptor Antagonist, Recombinant, Human (IL-1RA, IL1RA, IL-1ra3, IL-1RN,
IL1RN, Anakinra, DIRA, ICIL-1RA, IL1 Inhibitor, IL1F3, IRAP, MGC10430, MVCD4)

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Specifications

Interleukin-1 receptor antagonist (IL-1RA) is a naturally occurring protein which inhibits the activity of IL-1 alpha and IL-1 beta by competitively blocking binding to cell surface receptors for IL-1.
Catalog #I7663-25L3
ApplicationsSuitable for use in Functional Assays. Other applications not tested.
Endotoxin 0.1ng/ug
Biological ActivityIn an assay of dose dependent inhibition of IL-1 stimulation of D10S cells, the ED50 was expectedto be 20-40ng/ml.
Recommended DilutionFunctional Assays: 20-40ng/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight17kD
SourceRecombinant corresponding to human IL-1RA produced in E. coli
Purity~98% (SDS PAGE).
FormSupplied as a lyophilized powder from TBS. Reconstitute with 200ul sterile dH2O.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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