| Intracellular Interleukin 1 Receptor Antagonist (icIL-1Ra) is found in many cells as a constitutively expressed protein, particularly epithelial cells and keratinocytes. |
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| Biologically, secreted Interleukin 1 Receptor Antagonist (sIL-1Ra) and icIL-1Ra both function as IL-1 receptor antagonists by binding to the type I IL-1R and preventing the signaling by IL-1a or IL-1b. However, sIL-1Ra is primarily secreted whereas icIL-1Ra remains associated with the cell. |
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| Catalog # | I7663-25Q |
| Applications | Suitable for for use in Neutralization, ELISA, RIA, Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested. |
| Recommended Dilutions | ELISA: 1:500-1:1000; Using I1904-46Q and ABTS as a substrate for 30 minutes at RT. |
| RIA: 1:8000 |
| Western Blot: 1:400-1:800; Using I1904-46Q and TMB as a substrate. |
| Immunohistochemistry (paraffin-fixed/ frozen): 1:400 |
| Neutralization: 1:200 will neutralize the inhibitory activity of IL-1Ra at 100ng/ml |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | N/A |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added. |
| Purity | Purified by DEAE chromatography. |
| Endotoxin Content | < 10pg/ul by LAL method. |
| Immunogen | Recombinant human sIL-1Ra produced in E. coli. |
| Specificity | Recognizes human Interleukin 1 Receptor Antagonist. Detects primate IL-1Ra in the same formats using similar dilutions, but not mouse or rabbit IL-1Ra. Does not recognize human IL-1a, IL-1b or IL-1 receptor type I and type II. Primarily directed against the secreted form of IL-1Ra (also termed sIL-1Ra), but recognizes the native (non-denatured) as well as denatured intracellular form of IL-1Ra (also termed icIL-1Ra). |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
