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You are here:Home » Antibodies » Abs to Receptors » Anti -Interleukin 1 Receptor Antagonist (IL-1Ra)

Anti -Interleukin 1 Receptor Antagonist (IL-1Ra)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified E B IH FC RIA N
Intracellular Interleukin 1 Receptor Antagonist (icIL-1Ra) is found in many cells as a constitutively expressed protein, particularly epithelial cells and keratinocytes.
Biologically, secreted Interleukin 1 Receptor Antagonist (sIL-1Ra) and icIL-1Ra both function as IL-1 receptor antagonists by binding to the type I IL-1R and preventing the signaling by IL-1a or IL-1b. However, sIL-1Ra is primarily secreted whereas icIL-1Ra remains associated with the cell.
Catalog #I7663-25Q
ApplicationsSuitable for for use in Neutralization, ELISA, RIA, Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested.
Recommended DilutionsELISA: 1:500-1:1000; Using I1904-46Q and ABTS as a substrate for 30 minutes at RT.
RIA: 1:8000
Western Blot: 1:400-1:800; Using I1904-46Q and TMB as a substrate.
Immunohistochemistry (paraffin-fixed/ frozen): 1:400
Neutralization: 1:200 will neutralize the inhibitory activity of IL-1Ra at 100ng/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
PurityPurified by DEAE chromatography.
Endotoxin Content< 10pg/ul by LAL method.
ImmunogenRecombinant human sIL-1Ra produced in E. coli.
SpecificityRecognizes human Interleukin 1 Receptor Antagonist. Detects primate IL-1Ra in the same formats using similar dilutions, but not mouse or rabbit IL-1Ra. Does not recognize human IL-1a, IL-1b or IL-1 receptor type I and type II. Primarily directed against the secreted form of IL-1Ra (also termed sIL-1Ra), but recognizes the native (non-denatured) as well as denatured intracellular form of IL-1Ra (also termed icIL-1Ra).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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