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You are here:Home » Antibodies » Antibodies-Receptors » Anti -Interleukin 1 Receptor Antagonist (IL-1Ra)

Anti -Interleukin 1 Receptor Antagonist (IL-1Ra)


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Clone Host Grade Applications
Polyclonal Rabbit Highly Purified E B IH FC RIA N
Intracellular Interleukin 1 Receptor Antagonist (icIL-1Ra) is found in many cells as a constitutively expressed protein, particularly epithelial cells and keratinocytes.
Biologically, secreted Interleukin 1 Receptor Antagonist (sIL-1Ra) and icIL-1Ra both function as IL-1 receptor antagonists by binding to the type I IL-1R and preventing the signaling by IL-1a or IL-1b. However, sIL-1Ra is primarily secreted whereas icIL-1Ra remains associated with the cell.
Catalog #I7663-25Q
ApplicationsSuitable for for use in Neutralization, ELISA, RIA, Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested.
Recommended DilutionsELISA: 1:500-1:1000; Using I1904-46Q and ABTS as a substrate for 30 minutes at RT.
RIA: 1:8000
Western Blot: 1:400-1:800; Using I1904-46Q and TMB as a substrate.
Immunohistochemistry (paraffin-fixed/ frozen): 1:400
Neutralization: 1:200 will neutralize the inhibitory activity of IL-1Ra at 100ng/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
PurityPurified by DEAE chromatography.
Endotoxin Content< 10pg/ul by LAL method.
ImmunogenRecombinant human sIL-1Ra produced in E. coli.
SpecificityRecognizes human Interleukin 1 Receptor Antagonist. Detects primate IL-1Ra in the same formats using similar dilutions, but not mouse or rabbit IL-1Ra. Does not recognize human IL-1a, IL-1b or IL-1 receptor type I and type II. Primarily directed against the secreted form of IL-1Ra (also termed sIL-1Ra), but recognizes the native (non-denatured) as well as denatured intracellular form of IL-1Ra (also termed icIL-1Ra).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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