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You are here:Home » Growth Factors, Cytokines » Growth Factors-Interleukin » Interleukin 2, Recombinant, Mouse (IL-2)

Interleukin 2, Recombinant, Mouse (IL-2)

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Specifications

Recombinant Murine IL-2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 149 amino acids and having a molecular mass of 17231 Dalton. The Recombinant Murine IL-2 is purified by proprietary chromatographic techniques.
Catalog #I7663-26A1
AA SequencePTSSSTSSST AEAQQQQQQQ QQQQQHLEQL LMDLQELLSR MENYRNLKLP RMLTFKFYLP KQATELKDLQ CLEDELGPLR HVLDLTQSKS FQLEDAENFI SNIRVTVVKL KGSDNTFECQ FDDESATVVD FLRRWIAFCQ SIISTSPQ
Biological ActivityRecombinant Murine IL-2 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of murine CTLL-2 cells is < 0.2 ng/ml, corresponding to a Specific Activity of 5 MIU/mg.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.482 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IL-2 as a Reference Standard.
Molecular Weight17.23kD
SourceE. coli
Purity98% as determined by RP-HPLC, SDS-PAGE.
Concentration~1mg/ml
FormSupplied as a lyophilized powder in 10mM sodium citrate buffer, pH 4.0.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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