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Interleukin 2, Recombinant, Mouse (IL-2)
Interleukin 2, Recombinant, Mouse (IL-2)
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| Recombinant Murine IL-2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 149 amino acids and having a molecular mass of 17231 Dalton. The Recombinant Murine IL-2 is purified by proprietary chromatographic techniques. | | | Catalog # | I7663-26A1 | | AA Sequence | PTSSSTSSST AEAQQQQQQQ QQQQQHLEQL LMDLQELLSR MENYRNLKLP RMLTFKFYLP KQATELKDLQ CLEDELGPLR HVLDLTQSKS FQLEDAENFI SNIRVTVVKL KGSDNTFECQ FDDESATVVD FLRRWIAFCQ SIISTSPQ | | Biological Activity | Recombinant Murine IL-2 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of murine CTLL-2 cells is < 0.2 ng/ml, corresponding to a Specific Activity of 5 MIU/mg. | | Protein Content | Protein quantitation was carried out by two independent methods: | | 1. UV spectroscopy at 280 nm using the absorbency value of 0.482 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). | | 2. Analysis by RP-HPLC, using a calibrated solution of IL-2 as a Reference Standard. | | Molecular Weight | 17.23kD | | Source | E. coli | | Purity | 98% as determined by RP-HPLC, SDS-PAGE. | | Concentration | ~1mg/ml | | Form | Supplied as a lyophilized powder in 10mM sodium citrate buffer, pH 4.0. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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