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You are here:Home » Growth Factors, Cytokines » Growth Factors-Interleukin » Interleukin 2, Recombinant, Human (Interleukin-2, IL-2, IL2, Aldesleukin, Lymphokine, T-cell Growth Factor, TCGF)

Interleukin 2, Recombinant, Human (Interleukin-2, IL-2, IL2, Aldesleukin, Lymphokine,
T-cell Growth Factor, TCGF)

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Specifications

Recombinant corresponding to 134aa of human Interleukin-2 expressed in E.coli. I7663-26H is a single, non-glycosylated mutein (variant form) of human IL-2 polypeptide chain. I7663-26H has a Ser substitute for Cysteine at position 126.
Catalog #I7663-26H
Molecular Weight~15kD
Biological ActivityThe ED50 as determined by the dose-dependent stimulation of mouse CTLL-2 cells is less then 0.0645ng/ml, corresponding to a Specific Activity of 16.9 MIU/mg.
Dimers and Aggregates 1% (SDS-PAGE)
Protein ContentProtein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm using the absorbency value of 0.614 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of IL-2 as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile sterile 20mM acetic acid, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~15.5kD
SourceE.coli
Purity~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE)
Endotoxin0.1ng/ug (IEU/ug).
Concentration~1.1mg/ml
FormSupplied as a lyophilized powder. No preservative added. Reconstitute with sterile 20mM acetic acid, to 0.1mg/ml. For long term storage, add 0.1% HSA or BSA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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