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Interleukin 2, Recombinant, Human (Interleukin-2, IL-2, IL2, Aldesleukin, Lymphokine, T-cell Growth Factor, TCGF)
Interleukin 2, Recombinant, Human (Interleukin-2, IL-2, IL2, Aldesleukin, Lymphokine,
T-cell Growth Factor, TCGF)
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| IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. Recombinant Human IL-2 is a single, non-glycosylated mutein (variant form) of human IL-2 polypeptide chain containing 134 amino acids and having a molecular mass of 15.517kD. | | | Catalog # | I7663-26P | | AA Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Ser. | | Biological Activity | The ED50 as determined by the dose-dependent stimulation of murine CTLL-2 cells is | | < 0.0645ng/ml, corresponding to a Specific Activity of 15,500,000IU/mg. | | Protein Content | Protein quantitation was carried out by two independent methods: | | 1. UV spectroscopy at 280nm using the absorbency value of 0.614 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). | | 2. Analysis by RP-HPLC, using a calibrated solution of IL-2 as a Reference Standard. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 20mM acetic acid. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Source | E. coli | | Purity | ~98% by RP-HPLC and SDS-PAGE. Chromatographically purified. | | Concentration | Not determined | | Form | Supplied as a lyophilized powder from PBS, pH 7.5. Reconstitute in sterile 20mM acetic acid to a concentration of 0.1mg/ml. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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