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Specifications

Recombinant Rat IL-2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 134 amino acids and having a molecular mass of 15311 Dalton. The Recombinant Rat IL-2 is purified by proprietary chromatographic techniques.
Catalog #	I7663-28B
Amino Acid Sequence	The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Ser.
Dimers and Aggregates	1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity	Rat IL-2 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of murine CTLL-2 cells is < 0.4 ng/ml, corresponding to a Specific Activity of 2.5 MIU/mg.
Endotoxin	0.1ng/ug (IEU/ug) of Rat IL-2.
Protein Content	Protein quantitation was carried out by two independent methods:
	1. UV spectroscopy at 280 nm using the absorbency value of 0.535 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
	2. Analysis by RP-HPLC, using a calibrated solution of IL-2 as a Reference Standard.
Gene	Name: IL2
Solubility	It is recommended to reconstitute the lyophilized Recombinant Rat IL-2 in sterile 20mM AcOH not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability	Lyophilized Recombinant Rat IL-2 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Rat IL-2 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Molecular Weight	15.31kD
Source	E. coli
Purity	98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Concentration	~1mg/ml
Form	Supplied as a lyophilized powder in 10mM sodium citrate buffer, pH 4.0.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.