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You are here:Home » Molecular Biology » Growth Factors-Interleukin » Interleukin 2, Recombinant, Rat (IL-2)

Interleukin 2, Recombinant, Rat (IL-2)

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Specifications

Recombinant Rat IL-2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 134 amino acids and having a molecular mass of 15311 Dalton. The Recombinant Rat IL-2 is purified by proprietary chromatographic techniques.
Catalog #I7663-28B
Amino Acid SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Ser.
Dimers and Aggregates 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological ActivityRat IL-2 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of murine CTLL-2 cells is < 0.4 ng/ml, corresponding to a Specific Activity of 2.5 MIU/mg.
Endotoxin 0.1ng/ug (IEU/ug) of Rat IL-2.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.535 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IL-2 as a Reference Standard.
GeneName: IL2
SolubilityIt is recommended to reconstitute the lyophilized Recombinant Rat IL-2 in sterile 20mM AcOH not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
StabilityLyophilized Recombinant Rat IL-2 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Rat IL-2 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Molecular Weight15.31kD
SourceE. coli
Purity98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Concentration~1mg/ml
FormSupplied as a lyophilized powder in 10mM sodium citrate buffer, pH 4.0.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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