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You are here:Home » Growth Factors, Cytokines » Growth Factors-Interleukin » Interleukin 2, Recombinant, Porcine (IL-2)

Interleukin 2, Recombinant, Porcine (IL-2)

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Specifications

Recombinant Porcine IL-2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 134 amino acids and having a molecular mass of 15217 Dalton. Porcine IL-2 is purified by proprietary chromatographic techniques.
Catalog #I7663-28M1
Amino Acid SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Ser.
Dimers and AggregatesLess than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological ActivityPorcine IL-2 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of murine CTLL-2 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 10 MIU/mg.
Endotoxin 0.1ng/ug (IEU/ug) of Porcine IL-2.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.714 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IL-2 as a Reference Standard.
GeneName: IL2
SolubilityIt is recommended to reconstitute the lyophilized Porcine IL-2 in sterile 20mM AcOH not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
StabilityLyophilized Recombinant Porcine IL-2 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Porcine IL-2 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Molecular Weight15.21kD
SourceE. coli
Purity95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
ConcentrationAs reported
FormSupplied as a lyophilized powder without additives.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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